• First-generation (G1) isolate of Shiitake 75 (Aloha), a forgiving strain for a first wood-substrate grow
  • Colonizes grain in about 18 days at 62–75°F
  • Fruits across a wide 62–85°F range at 85–93% humidity; harvest about 20 days after fruiting conditions start
  • Best on supplemented hardwood sawdust or Master's Mix; let the block brown before fruiting
  • Includes a 16G 1.5" Luer Lock needle and alcohol prep pad; ships insulated
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Price: $12.50
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    Close-up of a clear liquid culture syringe labeled ‘Myco Labs Liquid Culture Syringe,’ positioned next to a dense block covered with clusters of brown shiitake mushrooms.

    Shiitake Liquid Culture Syringe, 10cc G1 Isolate (Lentinula edodes)

    Price: $12.50

    Our Shiitake is a first-generation Lentinula edodes isolate of the Shiitake 75 (Aloha) strain, chosen because it forgives mistakes. It colonizes rye berries well, fruits across an unusually wide temperature range and is the Shiitake we recommend for a first wood-substrate grow.

    • L. edodesSpecies
    • G1Isolated genetics
    • ~18 daysColonization
    • 62–85°FFruiting temp
    • BeginnerDifficulty

    Why grow our Shiitake culture

    Forgiving strain

    Shiitake 75 keeps performing in conditions that stall fussier strains, which makes it a good first wood-loving species.

    Wide fruiting range

    It fruits anywhere from 62 to 85°F, so a garage, basement or spare room works without a climate controller.

    Kitchen staple

    Deep, savory flavor that holds up to drying and rehydrating, and multiple flushes per block.

    Inoculating with liquid culture

    1Warm and shake

    Let the syringe reach room temperature, then shake it gently to break up and spread the mycelium through the broth.

    2Sterilize

    Flame the needle until it glows, let it cool a few seconds, and wipe the injection port with the included alcohol pad.

    3Inject

    Use 2–3cc per quart jar or 3–5cc per 3–5 lb grain bag. One 10cc syringe inoculates about 2–4 containers.

    4Incubate

    Keep it at 62–75°F in the dark. Shake once at about 30% colonization to speed things up.

    Wait for the brown: a colonized Shiitake block grows a tough, leathery brown skin before it fruits. That's normal. Let the whole block brown and firm up before you drop the temperature and add fresh air.

    Shiitake growing parameters

    SpecDetail
    Scientific nameLentinula edodes
    GeneticsFirst-generation (G1) isolated culture
    Recommended substratesSupplemented hardwood sawdust, Master's Mix (straw works but yields poorly)
    Incubation temperature62–75°F
    Incubation humidity80–85%
    Colonization timeAbout 18 days on grain
    Fruiting temperature62–85°F
    Fruiting humidity85–93%
    Time to harvestAbout 20 days after fruiting conditions start
    Fresh air exchangeModerate to high, 4–6 exchanges per hour
    In the package10cc syringe, 16G 1.5" Luer Lock needle, alcohol prep pad
    StorageRefrigerate at 35–45°F; never freeze

    From our lab to your grainRun it into jars with wide-mouth cultivation lids or your own lids with self-healing injection ports. Spare 16G needles and sterile Luer Lock syringes are sold separately.

    Frequently asked questions

    How much liquid culture do I use per jar or bag?

    Use 2–3cc per quart grain jar and 3–5cc per 3–5 lb spawn bag. One 10cc Shiitake syringe inoculates roughly two to four containers. Using more doesn't speed colonization much; it just uses up the syringe.

    How long does Shiitake take to colonize grain?

    About 18 days at 62–75°F, slower than oysters. After spawning to bulk, the block also needs time to brown before it will fruit, so plan for a longer cycle.

    What's the best substrate for Shiitake?

    Supplemented hardwood sawdust or Master's Mix, by a wide margin. Shiitake grows wild on oak, maple, beech and chestnut; straw technically works, but yields drop and the fruits come out thin.

    My Shiitake block turned brown. Is it contaminated?

    No. Browning is normal and required. The block forms a leathery brown skin before it fruits, and forcing it early gives poor flushes. Wait until it's evenly brown and firm.

    How should I store the syringe?

    Refrigerate it at 35–45°F in the bag it shipped in and never freeze it; freezing ruptures the cells and kills the culture. Bring it to room temperature and shake gently before you inject.

    Why liquid culture instead of a spore syringe?

    Liquid culture is living mycelium from an isolated culture, so it skips germination, colonizes days faster and gives genetically consistent fruits. Spores have to germinate and pair up first, which is slower and more variable from grow to grow.

    Packed to arrive alive

    Liquid culture is living mycelium, so every syringe leaves our lab in a foil-lined insulated mailer. The insulation buffers it against summer heat in a delivery truck and against sub-freezing winter temperatures, so it arrives viable either way.

    Myco Labs liquid culture syringe packed in a foil-lined insulated mailer

    When it arrives: refrigerate the syringe at 35–45°F until you're ready to inoculate. Never freeze it. Bring it to room temperature and shake gently before use.

    Overall Customer Rating of 2 Reviews:
    review-star review-star review-star review-star review-star
    Kentucky

    Excellent

    Rating:
    review-star review-star review-star review-star review-star
    Pros:
    Cons:
    Great genetics. Very healthy culture. Highly recommended.

    Great results

    Rating:
    review-star review-star review-star review-star review-star
    Pros:
    Cons:
    Very hardy. This has been my first working with Shiitake and it's been fantastic. Puts up with a couple noobie mistakes and bounces back.