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Culture Blend is the same liquid culture formula Myco Labs has used in our own lab for 15 years, pre-blended as a dry mix. Weigh it, dissolve it in hot water and pressure cook for sterile broth ready for spores, liquid culture or agar. One 250 g pouch makes more than 3 gallons.
Price: $19.99
Culture Blend is the formula behind every jar of liquid culture we've produced at Myco Labs for the last 15 years, pre-blended as a dry mix. Extra light malt extract, dextrose, soy peptone and our nutritional supplement and mineral blend come measured and mixed in one resealable pouch. Weigh it, dissolve it in hot water, pressure cook, and you have sterile broth ready for spores, liquid culture or an agar wedge. One 250 g pouch makes more than 3 US gallons.
No sourcing four separate ingredients, no working out ratios. The same blend, batch after batch.
Most homemade recipes are sugar and water. We add soy peptone and our nutritional supplement blend so mycelium has nitrogen to build with, not just sugar to burn.
At the label ratio, 250 g covers roughly 12 quart jars or 25 pint jars. Enough to keep a library of cultures going for a long while.
One blend and one weight per volume of water. When a culture performs well, you can make the exact same broth again.
All you add is water, jars with injection-port lids, and a pressure cooker.
Weigh the premix and stir it into hot water until no powder remains.
Pour the broth into your jars and leave headspace for air and swirling.
Pressure cook 30 minutes at 15 PSI. Going much longer darkens the sugars.
Once jars are at room temperature, add 3–5 cc of spore solution or liquid culture, or a clean agar wedge.
From our lab: pour the dissolved mix through a paper coffee filter before filling jars to take out malt sediment. Clearer broth makes new growth easier to spot and draws through a needle more easily. Incubate at 76–82°F out of direct light and swirl the jars regularly.
Powder packs down differently every time you scoop it, so spoon measurements change the strength of your broth from batch to batch. A gram scale takes that variable out. Use these amounts per volume of water:
Expect amber, not clear. Culture Blend is malt-based, so finished broth is yellow to light amber. That's normal, and it stays transparent enough to watch mycelium grow.
Need genetics?Culture Blend takes spores, liquid culture or agar. For spore syringes and prints, we recommend our friends at Spore Genetics and Premium Spores.
More than 3 US gallons at the label ratio of 19.4 g per quart. That's roughly 12 quart jars or 25 pint jars.
Weigh 9.7 g for each US pint of water, 19.4 g per US quart, 20.5 g per liter, or 77.5 g per US gallon. A gram scale gives you the same broth strength every time; measuring spoons don't.
Extra light malt extract, dextrose, soy peptone, and our proprietary nutritional supplement and mineral blend. It's the same formula Myco Labs has refined over 15 years of producing liquid cultures.
The malt extract gives the broth a yellow to light amber color, so it won't look like water, and that's expected. If it turns dark brown, it was cooked too long; stick to 30 minutes at 15 PSI.
It's optional. Running the dissolved mix through a paper coffee filter before filling jars removes malt sediment, which makes contamination and new growth easier to see and keeps the culture flowing smoothly through a needle.
30 minutes at 15 PSI. Let the jars cool completely to room temperature before you inoculate.
Spore solution, an existing liquid culture, or a clean wedge of colonized agar. For a jar, 3 to 5 cc of spore solution or liquid culture is plenty.
Keep the pouch sealed in a cool, dry place and use a clean, dry utensil every time you measure. The expiration date is printed on the back of each pouch. Culture Blend is for mushroom cultivation only and is not for human consumption.