Pioppino Liquid Culture Syringe, 10cc G1 Isolate

Pioppino (Black Poplar) Liquid Culture Syringe, 10cc G1 Isolate (Cyclocybe aegerita)

5.00 stars, based on 3 reviews 3 review(s). | Write a review

First-generation (G1) Cyclocybe aegerita isolate, the black poplar mushroom of Italian cooking
Colonizes grain in 16–20 days at 60–75°F
Fruits across a wide 55–75°F range at 90–93% humidity; harvest 18–21 days later
Peppery, earthy flavor with crisp, asparagus-like stems; beginner friendly
Includes a 16G 1.5" Luer Lock needle and alcohol prep pad; ships insulated

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Description
Reviews (3)

Our Pioppino is a first-generation Cyclocybe aegerita isolate, the black poplar mushroom of Italian kitchens. Clusters of brown, silky caps on firm, crisp stems carry a peppery, earthy, faintly floral flavor that holds its own in pasta and hearty sautés.

C. aegeritaSpecies
G1Isolated genetics
16–20 daysColonization
55–75°FFruiting temp
BeginnerDifficulty

Why grow our Pioppino culture

Flavor first

Sweet, peppery and earthy with a floral aroma as it cooks. It brings its own character instead of soaking up the pan.

Asparagus-crisp stems

The stems stay firm through cooking, so whole clusters go in stem and all.

Year-round fruiter

It fruits from 55 to 75°F, one of the widest ranges we carry, so a room that drifts with the seasons still produces.

Inoculating with liquid culture

1Warm and shake

Let the syringe reach room temperature, then shake it gently to break up and spread the mycelium through the broth.

2Sterilize

Flame the needle until it glows, let it cool a few seconds, and wipe the injection port with the included alcohol pad.

3Inject

Use 2–3cc per quart jar or 3–5cc per 3–5 lb grain bag. One 10cc syringe inoculates about 2–4 containers.

4Incubate

Keep it at 60–75°F in the dark. Shake once at about 30% colonization to speed things up.

Pick before the caps flatten: Pioppino drops a heavy load of dark brown spores once the caps open flat. Harvest while the edges are still rolled under to keep your chamber clean and the texture at its best.

Pioppino growing parameters

Spec	Detail
Scientific name	Cyclocybe aegerita
Genetics	First-generation (G1) isolated culture
Recommended substrates	Oats, milo, millet or rye for spawn; supplemented sawdust for bulk
Incubation temperature	60–75°F
Incubation humidity	80–85%
Colonization time	16–20 days on grain
Fruiting temperature	55–75°F
Fruiting humidity	90–93%
Time to harvest	18–21 days after fruiting conditions start
Spore drop	Heavy and dark brown; harvest before the caps flatten
In the package	10cc syringe, 16G 1.5" Luer Lock needle, alcohol prep pad
Storage	Refrigerate at 35–45°F; never freeze

From our lab to your grainRun it into jars with wide-mouth cultivation lids or your own lids with self-healing injection ports. Spare 16G needles and sterile Luer Lock syringes are sold separately.

Frequently asked questions

How much liquid culture do I use per jar or bag?

Use 2–3cc per quart grain jar and 3–5cc per 3–5 lb spawn bag. One 10cc Pioppino syringe inoculates roughly two to four containers. Using more doesn't speed colonization much; it just uses up the syringe.

How long does Pioppino take to colonize grain?

16–20 days at 60–75°F, then 18–21 days to harvest once fruiting conditions start.

What's the best substrate for Pioppino?

Rye, oats, milo or millet all work for spawn. For bulk, use supplemented hardwood sawdust; Pioppino is a wood lover and gives its best clusters on an enriched block.

Why is there brown dust all over my fruiting chamber?

Spores. Pioppino drops heavy dark-brown spore prints once the caps flatten. It's harmless, but it means you harvested late. Pick while the cap edges are still curled under.

How should I store the syringe?

Refrigerate it at 35–45°F in the bag it shipped in and never freeze it; freezing ruptures the cells and kills the culture. Bring it to room temperature and shake gently before you inject.

Why liquid culture instead of a spore syringe?

Liquid culture is living mycelium from an isolated culture, so it skips germination, colonizes days faster and gives genetically consistent fruits. Spores have to germinate and pair up first, which is slower and more variable from grow to grow.

Packed to arrive alive

Liquid culture is living mycelium, so every syringe leaves our lab in a foil-lined insulated mailer. The insulation buffers it against summer heat in a delivery truck and against sub-freezing winter temperatures, so it arrives viable either way.

Myco Labs liquid culture syringe packed in a foil-lined insulated mailer

When it arrives: refrigerate the syringe at 35–45°F until you're ready to inoculate. Never freeze it. Bring it to room temperature and shake gently before use.

Overall Customer Rating of 3 Reviews:

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Celeste

Louisiana

Info for the mushroom and mind

7/26/2025

Rating:

Pros:

Cons:

I love the fact that you guys share the good info like incubation info, substrate types and fruiting needs. So helpful!

DowneastBeast

Maine

Robust and pain-free experience

6/10/2025

Rating:

Pros:

Price
Shipping
Quality
Ease of cultivation

Cons:

Space requirements
Second flushes are challenging

Phenomenal grow experience. Never worked with these before. Inoculated via sorghum to a 5lb block of Masters Mix and incubated at room temp for 2 weeks+ until full colonization. Slit cut in bag below filter patch once introduced to fruiting conditions. After pins establish, widen the slit, and after fruit bodies are fully formed, cut from the slit and remove the top of the bag, leaving a 'collar' around the fruits to create a microclimate. AIO grow kit users should be familiar with the process.

Harvested 1 lean pound of product after 9 days, please see images.

MamaMia!

Nonstop Pinning!!!

5/4/2022

Rating:

Pros:

Cons:

I used these spores in my monotub, and I have never experienced such aggressive pinning before! The just mushrooms wouldn't stop coming! Tasted so yummy in my pasta dinner too :)

Pioppino (Black Poplar) Liquid Culture Syringe, 10cc G1 Isolate (Cyclocybe aegerita)

Why grow our Pioppino culture

Inoculating with liquid culture

Pioppino growing parameters

Frequently asked questions

Packed to arrive alive

Info for the mushroom and mind

Robust and pain-free experience

Nonstop Pinning!!!

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