• First-generation (G1) Pholiota adiposa isolate, chosen for reliable multi-flush yields
  • Colonizes grain in about 18 days at 65–75°F
  • Fruits at 50–70°F and 90–93% humidity; clusters mature 2–3 weeks after first pins
  • Nutty caps on stems that stay crunchy through cooking; beginner friendly
  • Includes a 16G 1.5" Luer Lock needle and alcohol prep pad; ships insulated
Retail: $19.99
Price: $12.50
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    Close-up of a clear liquid culture syringe labeled ‘Myco Labs Liquid Culture Syringe’ placed next to a dense cluster of golden-brown chestnut mushrooms growing on a substrate block

    Chestnut Mushroom Liquid Culture Syringe, 10cc G1 Isolate (Pholiota adiposa)

    Price: $12.50

    Our Chestnut is a first-generation Pholiota adiposa isolate chosen for reliability. It colonizes rye readily, accepts a range of bulk substrates and keeps producing flush after flush from the same block, which makes it one of the best yield-per-pound cultures we sell.

    • P. adiposaSpecies
    • G1Isolated genetics
    • ~18 daysColonization
    • 50–70°FFruiting temp
    • BeginnerDifficulty

    Why grow our Chestnut culture

    Flush after flush

    Where many species give one good round, Chestnut keeps coming back from the same block.

    Two textures, one pan

    Nutty, mild caps on stems that stay crunchy through cooking. Sauté whole clusters stem and all.

    Cool-season strain

    Fruits at 50–70°F alongside Blue Oyster and Shiitake, so it fits a fall and winter rotation.

    Inoculating with liquid culture

    1Warm and shake

    Let the syringe reach room temperature, then shake it gently to break up and spread the mycelium through the broth.

    2Sterilize

    Flame the needle until it glows, let it cool a few seconds, and wipe the injection port with the included alcohol pad.

    3Inject

    Use 2–3cc per quart jar or 3–5cc per 3–5 lb grain bag. One 10cc syringe inoculates about 2–4 containers.

    4Incubate

    Keep it at 65–75°F in the dark. Shake once at about 30% colonization to speed things up.

    Rehydrate between flushes: after each harvest, soak or mist the block to replace lost water, then return it to fruiting conditions. That's what keeps the later flushes coming.

    Chestnut growing parameters

    SpecDetail
    Scientific namePholiota adiposa
    GeneticsFirst-generation (G1) isolated culture
    Recommended substratesRye berries for spawn; supplemented sawdust or straw for bulk
    Incubation temperature65–75°F
    Incubation humidity80–85%
    Colonization timeAbout 18 days on grain
    Fruiting temperature50–70°F
    Fruiting humidity90–93%
    Time to harvestAbout 16 days; 2–3 weeks from first primordia
    FlushesReliably multi-flush; expect several rounds per block
    In the package10cc syringe, 16G 1.5" Luer Lock needle, alcohol prep pad
    StorageRefrigerate at 35–45°F; never freeze

    From our lab to your grainRun it into jars with wide-mouth cultivation lids or your own lids with self-healing injection ports. Spare 16G needles and sterile Luer Lock syringes are sold separately.

    Frequently asked questions

    How much liquid culture do I use per jar or bag?

    Use 2–3cc per quart grain jar and 3–5cc per 3–5 lb spawn bag. One 10cc Chestnut syringe inoculates roughly two to four containers. Using more doesn't speed colonization much; it just uses up the syringe.

    How long does Chestnut take to colonize grain?

    About 18 days at 65–75°F. Once the first primordia appear, clusters mature in about two to three weeks.

    What's the best substrate for Chestnut?

    Rye berries for spawn, then supplemented hardwood sawdust or straw for bulk. Supplemented sawdust gives the most flushes per block.

    How many flushes will Chestnut produce?

    Several. Chestnut is reliably multi-flush, so expect multiple rounds from one block when you rehydrate it between harvests. That's a big part of why it's a favorite for growers trying to get real food from limited substrate.

    How should I store the syringe?

    Refrigerate it at 35–45°F in the bag it shipped in and never freeze it; freezing ruptures the cells and kills the culture. Bring it to room temperature and shake gently before you inject.

    Why liquid culture instead of a spore syringe?

    Liquid culture is living mycelium from an isolated culture, so it skips germination, colonizes days faster and gives genetically consistent fruits. Spores have to germinate and pair up first, which is slower and more variable from grow to grow.

    Packed to arrive alive

    Liquid culture is living mycelium, so every syringe leaves our lab in a foil-lined insulated mailer. The insulation buffers it against summer heat in a delivery truck and against sub-freezing winter temperatures, so it arrives viable either way.

    Myco Labs liquid culture syringe packed in a foil-lined insulated mailer

    When it arrives: refrigerate the syringe at 35–45°F until you're ready to inoculate. Never freeze it. Bring it to room temperature and shake gently before use.

    Overall Customer Rating of 2 Reviews:
    review-star review-star review-star review-star review-star
    Wisconsin

    Great genetics!

    Rating:
    review-star review-star review-star review-star review-star
    Pros:
    Cons:
    Myco Labs never disappoints! I'm having great success with these cultures + their grain bags & masters mix substrate.

    Chestnuts

    Rating:
    review-star review-star review-star review-star review-star
    Pros:
    Cons:
    Injected into my bag and the grain was fully colonized in about 3 weeks. I spawned it to bulk, and it took about a week to fully colonize and it's ready to pin! I'll be buying all of my LC from Myco Lab.