• First-generation (G1) Pleurotus eryngii isolate, the largest oyster species, grown for its thick, meaty stem
  • Colonizes grain in about 12 days at 68–72°F
  • Fruits at 55–69°F and 90–93% humidity; harvest 18–44 days after fruiting conditions start
  • Needs heavy fresh air exchange; best on supplemented hardwood sawdust or Master's Mix
  • Includes a 16G 1.5" Luer Lock needle and alcohol prep pad; ships insulated
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    King Trumpet Liquid Culture Syringe, 10cc G1 Isolate (Pleurotus eryngii)

    Price: $12.50

    Our King Trumpet is a first-generation Pleurotus eryngii isolate, the largest oyster species and the only one grown for its stem. It forms thick, dense white stems topped with small tan caps, and a well-grown fruit can top two pounds.

    • P. eryngiiSpecies
    • G1Isolated genetics
    • ~12 daysColonization
    • 55–69°FFruiting temp
    • IntermediateDifficulty

    Why grow our King Trumpet culture

    Grown for the stem

    Thick white stems with a scallop-like texture. Sliced into rounds and seared, it's the most convincing meat substitute you can grow.

    Biggest oyster there is

    Single fruits can pass two pounds. Most home grows land in the quarter- to half-pound range, still far bigger than any other oyster.

    Cool-season grower

    It fruits at 55–69°F, so it pairs with Pink and Golden Oyster as the fall and winter strain.

    Inoculating with liquid culture

    1Warm and shake

    Let the syringe reach room temperature, then shake it gently to break up and spread the mycelium through the broth.

    2Sterilize

    Flame the needle until it glows, let it cool a few seconds, and wipe the injection port with the included alcohol pad.

    3Inject

    Use 2–3cc per quart jar or 3–5cc per 3–5 lb grain bag. One 10cc syringe inoculates about 2–4 containers.

    4Incubate

    Keep it at 68–72°F in the dark. Shake once at about 30% colonization to speed things up.

    Airflow makes or breaks it: King Trumpet is more CO2-sensitive than any other oyster. Give it more fresh air than you would Blue or Pearl, or you'll get tall, pale stems with tiny caps.

    King Trumpet growing parameters

    SpecDetail
    Scientific namePleurotus eryngii
    GeneticsFirst-generation (G1) isolated culture
    Recommended substratesSupplemented hardwood sawdust, Master's Mix, straw
    Incubation temperature68–72°F
    Incubation humidity80–85%
    Colonization timeAbout 12 days on grain
    Fruiting temperature55–69°F
    Fruiting humidity90–93%
    Time to harvest18–44 days after fruiting conditions start
    Fresh air exchangeCritical; high CO2 stretches stems and shrinks caps
    In the package10cc syringe, 16G 1.5" Luer Lock needle, alcohol prep pad
    StorageRefrigerate at 35–45°F; never freeze

    From our lab to your grainRun it into jars with wide-mouth cultivation lids or your own lids with self-healing injection ports. Spare 16G needles and sterile Luer Lock syringes are sold separately.

    Frequently asked questions

    How much liquid culture do I use per jar or bag?

    Use 2–3cc per quart grain jar and 3–5cc per 3–5 lb spawn bag. One 10cc King Trumpet syringe inoculates roughly two to four containers. Using more doesn't speed colonization much; it just uses up the syringe.

    How long does King Trumpet take to colonize grain?

    About 12 days at 68–72°F. Fruiting is slower and less predictable than other oysters, anywhere from 18 to 44 days after conditions start.

    What's the best substrate for King Trumpet?

    Supplemented hardwood sawdust or Master's Mix gives the thickest, heaviest stems. Straw works, but the extra nutrition in a supplemented block is where the size comes from.

    Why are my King Trumpets tall and thin with tiny caps?

    Carbon dioxide. In stale air the stems stretch while the caps stall. Increase fresh air exchange well beyond what you'd use for other oysters and the fruits come in short, thick and heavy.

    How should I store the syringe?

    Refrigerate it at 35–45°F in the bag it shipped in and never freeze it; freezing ruptures the cells and kills the culture. Bring it to room temperature and shake gently before you inject.

    Why liquid culture instead of a spore syringe?

    Liquid culture is living mycelium from an isolated culture, so it skips germination, colonizes days faster and gives genetically consistent fruits. Spores have to germinate and pair up first, which is slower and more variable from grow to grow.

    Packed to arrive alive

    Liquid culture is living mycelium, so every syringe leaves our lab in a foil-lined insulated mailer. The insulation buffers it against summer heat in a delivery truck and against sub-freezing winter temperatures, so it arrives viable either way.

    Myco Labs liquid culture syringe packed in a foil-lined insulated mailer

    When it arrives: refrigerate the syringe at 35–45°F until you're ready to inoculate. Never freeze it. Bring it to room temperature and shake gently before use.

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    I continue to purchase my liquid cultures from Midwest. The selection is great, the shipping and prices are affordable…the customer service is terrific too.